Bioremediation and metagenomics applied to the decontamination of mangrove sediments

CNPq/MCTI - Research and Development for Addressing Oil Spills Along the Brazilian Coast – Science at Sea Program - No. 06/2020

About the Project

Coordinator

Prof. Dra. Vania Aparecida Vicente

Federal University of Paraná (UFPR)

Thematic Line

Bioremediation and physicochemical remediation applied to heavy oils

Summary

The proposed project involves the implementation of biotechnology-based strategies with the aim of mitigating the impacts of oil and accelerating its degradation. The application of taxonomic and functional metagenomics is employed to assess microbial biodegradation potential.

Description

In 2019, a major crude oil spill was detected along the coast of northeastern Brazil. The oil slicks affected extensive stretches of beach and caused damage to marine life and the health of the local population. This project establishes biotechnology-based environmental mitigation measures to reduce the impacts of the spill and accelerate the degradation of these components. In addition, the application of taxonomic and functional metagenomics to identify microbial potential for biodegradation and select promising microorganisms for environmental oil decontamination.

Objectives

General Objective

The project aims to assess the effects of oil contamination on mangroves in Sergipe, Brazil. This will be achieved by developing environmental monitoring protocols to evaluate the impact of the accident on the microbiota, and to evaluate the decontamination potential of the native microbiota, the use of biosurfactants, and their effects on contaminated sediments.

Methodology
Mangrove Oil Spill

Specific Objectives

  1. Characterize the microbial community in the study environment using functional and phylogenetic metagenomics;
  2. Isolate and screen biosurfactant-producing microorganisms and to assess the potential of bioremediating species;
  3. Enrich biosurfactant-degrading prokaryotes under aerobic and anaerobic conditions;
  4. Produce biosurfactants by indigenous microorganisms;
  1. Characterize the biosurfactant potential of indigenous microorganisms in microcosms;
  2. Evaluate the functional capacity and diversity of the microbial community in microcosms under different treatments;
  3. Establish a bioremediation protocol based on microcosm treatments;
  4. Test the applicability of biosurfactants and bioremediators in the field (mesocosms).

Executing Team

Managers - National Focal Groups

Prof. V. A. Vicente

Prof. Dra. Vania Aparecida Vicente

Coordinator

Walter Antonio Pereira Boeger

Walter Antonio Pereira Boeger

Oceanologist, Doctorate in Zoology
Collections of Microorganisms, Laboratory of Biological Interactions – UFPR

Derlene Attili de Angelis

Derlene Attili de Angelis

Biologist, PhD in Biological Sciences (Plant Biology)
Curator of the Brazilian Collection of Environmental and Industrial Microorganisms – CBMAI/UNICAMP

Valéria Maia de Oliveira

Valéria Maia de Oliveira

Biologist, PhD in Genetics of Microorganisms and Molecular Biology (IB/UNICAMP). Researcher at DRM.

Dr. Marlon Roger Geraldo

Dr. Marlon Roger Geraldo

Biologist, Doctor in Microbiology, Parasitology and Pathology

Jessica

Jessica

CNPq Industrial Technological Development Fellow

Marcelo Acacio Chammas

Marcelo Acacio Chammas

Technical Manager at Aquatrix Consulting and Projects

Financial Support

Financial Support

Contact

Principal Coordinator

Prof. Dra. Vania Aparecida Vicente

Federal University of Paraná (UFPR)

Department of Basic Pathology

Participating Institutions

This project is a collaboration between multiple Brazilian and international institutions, funded by CNPq/MCTI/CT-Health.